Comparison of direct and indirect fluorescent antibody methods for staining Treponema pallidum.
نویسندگان
چکیده
منابع مشابه
Specific immunofluorescence staining of Treponema pallidum in smears and tissues.
To date, tissue sections prepared from Formalin-fixed tissues have not been successfully stained with Treponema pallidum subspecies-specific antibody in a direct fluorescent-antibody assay. While current methods stain T. pallidum, they do not distinguish T. pallidum from other spirochetes such as Borrelia burgdorferi (E. F. Hunter, P. W. Greer, B. L. Swisher, A. R. Simons, C. E. Farshy, J. A. C...
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SUMMARY The results of direct stool examination in 102 watery and bloody diarrhea patients were compared with the presence of antibodies against Entamoeba histolytica with Indirect Fluorescent Antibody Test. Besides 22 healthy individuals included in this study as control group. It was found that 22 patients (21.5%) with a sera titre higher than 1/320 bad only 9 (8.8%) positive E. Histolytica ...
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It has been shown by our experiments that the serum of rabbits treated with emulsions of Treponema pallidum contains agglutinating substances. Normal rabbit serum also possesses agglutinating power for this organism, but, as in the case of normal bacterial agglutinins, to an extent very much inferior to that possessed by the sera of immunized animals. Normal human sera will agglutinate similar ...
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This is the first report of the fluorescent antibody tissue stain for Treponema pallidum in the eye. The fluorescent antibody (FA) technique was first described in 1942 by Coons as a means of identifying pneumococcal antigens in tissue. It has subse quently been applied to identification of other bacteria and their antigens." The fluorescent antibody technique was applied to syphilis by Deacon...
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The intralobular distribution of nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c (P-450) reductase (NADPH:ferricytochrome oxidoreductase, EC 1.6.2.4) in rat liver has been investigated by means of two quantitative immunohistochemical techniques: microdensitometric quantitation of unlabeled antibody peroxidaseantiperoxidase staining and microfluorometric analysis of indirect flu...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1971
ISSN: 1368-4973
DOI: 10.1136/sti.47.4.255