Comparison between Four Types of Buffers for DNA Agarose Gel Electrophoresis
نویسندگان
چکیده
To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, buffers were used widely to separate any DNA molecule. In this paper, four types previously known compare which is easy for preparation, simple in structure, low cost good performance agarose gel electrophoresis. For this, two concentration (1%, 2%) ladder (100 bp, 1 kb) represent both smaller larger sizes molecule each type buffers, from result found first level supper TAE with second bicarbonate also also. Finally, tang cannot pose electrophoretic activity on
منابع مشابه
[Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification.].
BACKGROUND Real-time polymerase chain reaction (PCR) is generally regarded as a very accurate and time-saving method, but it is expensive to run. We evaluated the reliability of an inexpensive and a researcher-friendly gel electrophoresis-based PCR method for the quantification of mRNA, and the results were compared with those obtained by real-time PCR. METHODS We compared the results of rela...
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متن کاملAgarose gel electrophoresis.
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Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose) subunits(2). During gelation, agarose polymers associate non-covalently and form a network of bundles whose pore sizes determine a gel's mo...
متن کاملAlkaline agarose gel electrophoresis.
INTRODUCTION Alkaline agarose gels are run at a pH that is sufficiently high to denature double-stranded DNA. The denatured DNA is maintained in a single-stranded state and migrates through the alkaline gel as a function of its size. Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand c...
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ژورنال
عنوان ژورنال: Journal of Biomaterials and Nanobiotechnology
سال: 2023
ISSN: ['2158-7027', '2158-7043']
DOI: https://doi.org/10.4236/jbnb.2023.143004