Comparison between a soluble antigen-based ELISA and IFAT in detecting antibodies against Babesia canis in dogs

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Comparison between a soluble antigen-based ELISA and IFAT in detecting antibodies against Babesia canis in dogs.

An available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-B. canis antibodies in the sera of dogs using, indirect fluorescent antibody test (IFAT) as a reference test. ELISA uses a soluble antigenic preparation of B. canis and the optimal dilutions of the antigen, serum and conjugate were determined by check board titration, using positive and negative referen...

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Leishmania, Babesia and Ehrlichia in urban pet dogs: co-infection or cross-reaction in serological methods?

INTRODUCTION The present study was designed to assess the occurrence of co-infection or cross-reaction in the serological techniques used for detecting the anti-Leishmania spp., -Babesia canis vogeli and -Ehrlichia canis antibodies in urban dogs from an area endemic to these parasites. METHODS The serum samples from dogs were tested for the Babesia canis vogeli strain Belo Horizonte antigen a...

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Autochthonous Babesia canis, Hepatozoon canis and imported Babesia gibsoni infection in dogs in the Czech Republic

The epidemiology of tick-borne diseases as well as the ecology of the associated tick vectors is currently undergoing dynamic change. Canine babesiosis has, to date, only been reported in the Czech Republic as an imported disease, despite the presence of an established population of D. reticulatus in the South Moravian region. A recent report of autochthonous H. canis in dogs indicates that the...

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Babesia canis canis and Babesia canis

Aim: A study on the clinical and biochemical parameters of Nigerian dogs experimentally infected with Babesia canis was conducted. Materials and Methods: A total of ten naive dogs of both sex and aged between 6 months to 1 year, were used for the study. They were divided into two groups of five eachA (control) and B (infected). Dogs in group B were infected with 1ml of Babesia canis positive in...

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A comparison of two antigen-detection ELISA for detecting infection of Dirofilaria immitis in dogs.

A survey on 87 dogs necropsied in the Townsville region revealed 34 (39%) were infected with Dirofilaria immitis. Infected dogs had an average of 6.1 adult worms in the heart and associated blood vessels. The VetRED assay detected 23 of the 34 infected dogs (sensitivity 65%) and the Og4C3 ELISA detected 27 (sensitivity 80%). Sensitivity of the VetRED and Og4C3 ELISA increased to 88 and 94% resp...

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ژورنال

عنوان ژورنال: Revista Brasileira de Parasitologia Veterinária

سال: 2009

ISSN: 1984-2961

DOI: 10.4322/rbpv.01803007