Comparative studies on the liver sulphatases

672 Studies on Sulphatases

In the preceding paper (Dodgson & Powell, 1959) it was shown that an arylsulphatase present in the digestive juice and the digestive gland of the edible snail exhibited a shift in optimum pH as the concentration of substrate was increased. This paper describes the partial purification of the digestivegland enzyme and provides further evidence that the shift in optimum pH is not due to the prese...

Studies on sulphatases. V. The determination of inorganic sulphate in the study of sulphatases.

59 Studies on Sulphatases 9 . THE ARYLSULPHATASES OF MAMMALIAN LIVERS

Kinetic data for the hydrolysis of potassium pacetylphenyl sulphate by fresh rat liver suspensions suggested that the arylsulphatase activity so observed could be attributed to a single enzyme (Dodgson, Spencer & Thomas, 1953) which was later found to be localized mainly in the microsomes of the liver cell (Dodgson, Spencer & Thomas, 1954a). Preliminary attempts to purify the microsome enzyme (...

Comparative immunological studies in liver diseases.

By fractionation of human liver tissue, nuclear, mitochondrial, and connective tissue antigens were prepared. According to the results of serological tests, in conditions associated with substantial damage to the hepatic parenchyma, antibodies reacting with the nuclear and mitochondrial antigens are produced that show practically no reaction with the connective tissue antigen. It is advisable t...

Studies on sulphatases. 4. Arylsulphatase and beta-glucuronidase concentrates from limpets.