Comparative RNA expression analyses from small-scale, single-donor platelet samples1

Single donor platelet transfusion.

Cytopheresis equipment from a number of manufacturers now permits the rapid, safe, and efficient procurement of functionally normal platelets from single donors. There has been an extensive proliferation of these blood cell separators and virtually all large blood collection centers and most large hospitals have at least one blood cell separator in operation. Transfusions from single donors sel...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Refractoriness to platelet transfusion after single-donor consecutive platelet transfusions and its relationship to platelet antibodies.

In thirty patients with acute leukemia and severe aplastic anemia receiving random single donor platelet transfusions, the development of refractoriness by consecutive platelet transfusions with cytapheresis and its relationship to the appearance of anti-platelet antibodies were investigated. The median number of platelet transfusions inducing refractoriness was 13 times, and 20% of the patient...

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

Small RNA Expression from the Human Macrosatellite DXZ4

Small noncoding RNAs play several roles in regulating gene expression. In the nucleus, small RNA-Argonaute complexes recruit epigenetic modifying activities to genomic sites. This pathway has been described in mammals primarily for the germline; however, its role in somatic cells is less characterized. Here, we describe in human somatic cells a potential link between the expression of small RNA...