Comparative proteomic analysis to identify the novel target gene of angiotensin II in adrenocortical H295R cells

Angiotensin II (Ang II) is a well-known peptide that maintains the balance of electrolytes in higher vertebrates. Ang stimulation adrenal gland induces synthesis mineralocorticoids, mainly aldosterone, through up-regulation aldosterone synthase (CYP11B2) gene expression. Additionally, it has been reported activates multiple signaling pathways such as mitogen-activated protein kinase (MAPK) and Ca2+ signaling. Although various effects on cellular cells, its biological significance, except for synthesis, still unclear. In this study, we attempted to search novel target gene(s) human H295R cells using proteomic approach combined with stable isotopic labeling amino acid cell culture (SILAC). Interestingly, found elevated expression phosphofructokinase type platelet (PFKP) both mRNA levels. Moreover, transactivation PFKP by was dependent extracellular-signal-regulated (ERK) 1/2 activation. Finally, observed treatment facilitated glucose uptake cells. Taken together, here identified II, indicating not only stimulates steroidogenesis but also affects metabolism.