Comparative Genome Analysis of Two Heterotrophic Nitrifying Pseudomonas putida Strains Isolated from Freshwater Shrimp Ponds in Soc Trang Province

Nitrogen compounds, especially ammonia, are widely produced in aquaculture systems during cultivation. Ammonia has been investigated as a model compound for use by heterotrophic nitrifying bacteria. Pseudomonas TT321 and TT322, isolated from shrimp pond water Soc Trang province, Vietnam, identified comparing them with 31 of the closest genomes sequences NCBI nucleotide database. The genome sizes strains TT322 were 5,566,241 bp 5,563,644 bp, respectively. No plasmids evident these strains. Genome analysis revealed that belonged to putida shared common ancestor 33 genomes. Analysis based on comparison showed three genes, carbamate kinase (arcC), glutamine synthetase (Glul), aminomethyltransferase (amt), involved metabolic pathways. These pathways are: (i) arginine proline metabolism, (ii) alanine, aspartate glutamate (iii) glycine, serine threonine metabolism. genes may play important roles ammonia reduction support bacterial growth via assimilation.