Comparative Determination of Melittin by Capillary Electrophoretic Methods

Bee venom from honey bees (Apis Mellifera L.) is known to have many pharmacological and biological properties. Melittin, a peptide consisting of 26 amino acids, as the main component bee venom. The study aims develop rapid capillary electrophoresis method for separating quantifying melittin in honeybee Since basic peptide, it will adhere wall during separation. Two different methods were developed this electrophoretic separation melittin. As first approach, low pH buffer system was used. For second column coated with positively charged polymer (PEI). With both developed, migration achieved by preventing adsorption. Melittin migrated 6 min when low-pH applied, whereas its time longer than 10 PEI-coated column. Thus, preferred analysis actual bee-venom sample. 100 mmol L-1 phosphoric acid/sodium dihydrogen phosphate at 1.55 chosen buffer. conclusion, fast reliable determination applied an Anatolian sample highlight amount. amount found 24.5 ± 3.4 g g-1