Comparative Analysis of Expressed Genes from Cacao Meristems Infected by Moniliophthora perniciosa
نویسندگان
چکیده
منابع مشابه
Comparative analysis of expressed genes from cacao meristems infected by Moniliophthora perniciosa.
BACKGROUND AND AIMS Witches' broom disease is caused by the hemibiotrophic basidiomycete Moniliophthora perniciosa, and is one of the most important diseases of cacao in the western hemisphere. Because very little is known about the global process of such disease development, expressed sequence tags (ESTs) were used to identify genes expressed during the Theobroma cacao-Moniliophthora pernicios...
متن کاملProtein extraction for proteome analysis from cacao leaves and meristems, organs infected by Moniliophthora perniciosa, the causal agent of the witches' broom disease.
Preparation of high-quality proteins from cacao vegetative organs is difficult due to very high endogenous levels of polysaccharides and polyphenols. In order to establish a routine procedure for the application of proteomic and biochemical analysis to cacao tissues, three new protocols were developed; one for apoplastic washing fluid (AWF) extraction, and two for protein extraction--under dena...
متن کاملNecrotrophic phase of Moniliophthora perniciosa causes salicylic acid accumulation in infected stems of cacao
Moniliophthora perniciosa infects young meristematic tissue of Theobroma cacao and induces abnormal shoot development and ultimately necrosis. We analyzed culture filtrates of M. perniciosa to identify metabolites with possible effects on witches’ broom disease symptom development. All isolates produced salicylic acid (SA), phenyllactic acid and mandelic acid. When SA levels were measured in pl...
متن کاملExpression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae).
Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches' broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regu...
متن کاملThe hemibiotrophic cacao pathogen Moniliophthora perniciosa depends on a mitochondrial alternative oxidase for biotrophic development
The tropical pathogen Moniliophthora perniciosa causes witches' broom disease in cacao. As a hemibiotrophic fungus, it initially colonizes the living host tissues (biotrophic phase), and later grows over the dead plant (necrotrophic phase). Little is known about the mechanisms that promote these distinct fungal phases or mediate the transition between them. An alternative oxidase gene (Mp-aox) ...
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ژورنال
عنوان ژورنال: Annals of Botany
سال: 2007
ISSN: 0305-7364,1095-8290
DOI: 10.1093/aob/mcm092