Community analyses of sulfidogenic 2-bromophenol-dehalogenating and phenol-degrading microbial consortia
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چکیده
منابع مشابه
Detection by PCR of reductive dehalogenase motifs in a sulfidogenic 2-bromophenol-degrading consortium enriched from estuarine sediment.
Abstract Polymerase chain reaction (PCR) primers were developed based on three known reductive dehalogenase (RDH) genes (pceA from Dehalospirillum multivorans, cprA from Desulfitobacterium dehalogenans, tceA from Dehalococcoides ethenogenes) and used to amplify bands of the appropriate size from a microbial consortium inoculated with contaminated estuarine sediment and enriched under sulfidogen...
متن کاملDetection and characterization of a dehalogenating microorganism by terminal restriction fragment length polymorphism fingerprinting of 16S rRNA in a sulfidogenic, 2-bromophenol-utilizing enrichment.
Terminal restriction fragment length polymorphism analysis of reverse-transcribed 16S rRNA during periods of community flux was used as a tool to delineate the roles of the members of a 2-bromophenol-degrading, sulfate-reducing consortium. Starved, washed cultures were amended with 2-bromophenol plus sulfate, 2-bromophenol plus hydrogen, phenol plus sulfate, or phenol with no electron acceptor ...
متن کاملMolecular analysis of phenol-degrading microbial strains.
In an attempt to estimate the occurrence of phenol hydroxylase-related gene sequences we performed a dot blot hybridization assay with DNA from phenol utilizing Trichosporon cutaneum R57 strain NBIMCC 2414 and microbial isolates from different wastewaters. The used oligonucletides were homologous to the 5'-end of TORPHD locus (NCBI)-coding phenol hydroxylase in Trichosporon cutaneum ATCC 46490 ...
متن کاملCommunity and proteomic analysis of methanogenic consortia degrading terephthalate.
Degradation of terephthalate (TA) through microbial syntrophy under moderately thermophilic (46 to 50°C) methanogenic conditions was characterized by using a metagenomic approach (A. Lykidis et al., ISME J. 5:122-130, 2011). To further study the activities of key microorganisms responsible for the TA degradation, community analysis and shotgun proteomics were used. The results of hierarchical o...
متن کاملMetataxonomic profiling and prediction of functional behaviour of wheat straw degrading microbial consortia
BACKGROUND Mixed microbial cultures, in which bacteria and fungi interact, have been proposed as an efficient way to deconstruct plant waste. The characterization of specific microbial consortia could be the starting point for novel biotechnological applications related to the efficient conversion of lignocellulose to cello-oligosaccharides, plastics and/or biofuels. Here, the diversity, compos...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 1999
ISSN: 0168-6496,1574-6941
DOI: 10.1111/j.1574-6941.1999.tb00605.x