Common garlic (Allium sativum L.) has potent Anti-Bacillus anthracis activity

Gastrointestinal anthrax, a disease caused by Bacillus anthracis, remains an important but relatively neglected endemic of animals and humans in remote areas the Indian subcontinent some parts Africa. Its initial symptoms include diarrhea stomachache. In current study, several common plants indicated for diarrhea, dysentery, stomachache or as stomachic per traditional knowledge subcontinent, i.e., Aegle marmelos (L.) Correa (Bael), Allium cepa L. (Onion), sativum (Garlic), Azadirachta indica A. Juss. (Neem), Berberis asiatica Roxb. ex DC. (Daruharidra), Coriandrum (Coriander), Curcuma longa (Turmeric), Cynodon dactylon Pers. (Bermuda grass), Mangifera (Mango), Morus (Black mulberry), Ocimum tenuiflorum (Ocimum sanctum L., Holy Basil), gratissimum (Ram Tulsi), Psidium guajava (Guava), Zingiber officinale Roscoe (Ginger), were evaluated their anti-Bacillus anthracis property. The usage Santals (India), sp. biblical people to alleviate anthrax-like is well documented, other traditionally only different gastrointestinal disturbances/conditions. Evaluate above listed commonly available edible from that are used medicine treat diseases including those also presence potent anti-B. activity form amenable use general population areas. Aqueous extracts made fourteen screened using agar-well diffusion assay (AWDA) broth microdilution methods. Garlic Extract (AGE) displayed most was assessed its thermostability, stability under pH extremes encountered tract, potential antagonistic interaction with bile salts FDA-approved antibiotics anthrax control. bioactive fractions AGE isolated TLC coupled bioautography followed characterization GC-MS. (Allium L.) extract identified promising candidate bactericidal against B. anthracis. It consistently inhibited growth AWDA decreased viable colony-forming unit counts liquid-broth cultures 6-logs within 6–12 h. acceptable thermostability (>80% retained on incubation at 50 °C 12 h) gastric range (2–8). did not antagonize GC-MS analysis separated previously unreported constituents such phthalic acid derivatives, esters, phenyl group-containing compounds, steroids etc. activity. better than (Neem) while (Turmeric) show any conditions used. Further work should be undertaken explore possible application preventing incidences