Commentary: Genetic modification through oligonucleotide-mediated mutagenesis. A GMO regulatory challenge?
نویسندگان
چکیده
منابع مشابه
Genetic modification through oligonucleotide-mediated mutagenesis. A GMO regulatory challenge?
In the European Union, the definition of a GMO is technology-based. This means that a novel organism will be regulated under the GMO regulatory framework only if it has been developed with the use of defined techniques. This approach is now challenged with the emergence of new techniques. In this paper, we describe regulatory and safety issues associated with the use of oligonucleotide-mediated...
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The expression of Beta, the single-stranded annealing protein (SSAP) of bacteriophage lambda in Escherichia coli promotes high levels of oligonucleotide (oligo)-mediated mutagenesis and offers a quick way to create single or multiple base pair insertions, deletions, or substitutions in the bacterial chromosome. High rates of mutagenesis can be obtained by the use of mismatch repair (MMR)-resist...
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Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is emerging as a powerful tool for the introduction of subtle gene modifications in mouse embryonic stem (ES) cells and the generation of mutant mice. Here, we have studied the role of ssODN composition, transcription and replication of the target locus, and DNA repair pathways to gain more insight into the parameters governin...
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ژورنال
عنوان ژورنال: Environmental Biosafety Research
سال: 2009
ISSN: 1635-7922,1635-7930
DOI: 10.1051/ebr/2009007