Combining Suppression Subtractive Hybridization and Microarrays To Map the Intraspecies Phylogeny of Flavobacterium psychrophilum
نویسندگان
چکیده
منابع مشابه
Suppression subtractive hybridization.
Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...
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Suppression subtractive hybridization (SSH) is frequently used to unearth differentially expressed genes on a whole-genome scale. Its versatility is based on combining cDNA library subtraction and normalization, which allows the isolation of sequences of varying degrees of abundance and differential expression. SSH is a complex process with many adjustable parameters that affect the outcome of ...
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16 Routine application of antimicrobials is the current treatment of choice for rainbow trout 17 fry syndrome (RTFS) or bacterial coldwater disease (BCWD) caused by Flavobacterium 18 psychrophilum. In this study, the antimicrobial susceptibilities of 133 F. psychrophilum 19 isolates, 118 of which were from the UK, were evaluated by broth microdilution and disc 20 diffusion methods following VET...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2005
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.73.6.3799-3802.2005