Combining multiple markers significantly increases the sensitivity and precision of <scp>eDNA</scp> ‐based single‐species analyses
نویسندگان
چکیده
The analysis of environmental DNA (eDNA) is becoming integrated as an established biomonitoring tool, often characterized by detection limits exceeding those conventional counterparts. However, further improving the sensitivity these methods may be invaluable for early invasive species, or locating remnant populations endangered and adequate quantification their abundances. In this study, we provide empirical evidence showing that implementation multiple genetic markers targeting different loci a surprisingly overlooked strategy to increase single-species detections abundance, particularly at lower end species abundance range. We analyzed 45 natural eDNA samples obtained from wide range water bodies in Belgium, which either American bullfrog (Lithobates catesbeianus) rare European weather loach (Misgurnus fossilis) occurred under variable abundances, compared success precision simplex (single locus) versus multiplex (multilocus) droplet digital PCR (ddPCR) analyses. Multiplexing primer/probe assays independent resulted significantly enhanced probability analyses, gaining twofold reduction limit (LOD). Also improved reactions, especially low concentration samples. This was reflected significant coefficient variation (CV) among technical replicates, resulting associated decrease (LOQ). conclude use can improve analytical eDNA-based absolute quantifications.
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ژورنال
عنوان ژورنال: Environmental DNA
سال: 2023
ISSN: ['2637-4943']
DOI: https://doi.org/10.1002/edn3.420