Combined Small RNA and Degradome Sequencing Reveals Important Roles of Light-Responsive microRNAs in Wild Potato (Solanum chacoense)

The accumulation of chlorophyll and antinutritional glycoalkaloids in potato tubers resulting from exposure to light has been widely recognized as a cause unpredictable quality loss tuber. While transcriptional regulation light-induced extensively investigated, the mechanisms post-transcriptional through miRNA remain largely unexplored. An experimental model, Solanum chacoense, were used identify light-responsive miRNA–target interactions (MTIs) related tuber greening glycoalkaloid biosynthesis by employing multi-omics approaches (miRNA-seq degradome-seq). A total 732 unique mature miRNAs have identified S. chacoense. In total, 6335 target transcripts cleaved 489 known 153 novel miRNAs. results revealed that can be grouped into eight temporally clusters play important roles various physiological processes such plant growth, stress responses, primary secondary metabolism. Multi-omics analyses modulation transcript abundance MYB59, HSPs, EBF1/EBF2 is pivotal for their function cross-tolerance responses both abiotic biotic stresses. Furthermore, our findings suggest many are crucial regulators biosynthetic pathways, including tetrapyrrole biosynthesis, suberin steroid biosynthesis. These highlight significant role metabolic particularly isoprenoid, terpenoid, implications precise manipulation pathways produce new varieties with improved resistance lower levels.