Combination of Proteins with Polyelectrolytes Ions and Conductometric Titration of Polyelectrolytes.

Complex coacervation of supercharged proteins with polyelectrolytes.

Complexation of proteins with polyelectrolytes or block copolymers can lead to phase separation to generate a coacervate phase or self-assembly of coacervate core micelles. However, many proteins do not coacervate at conditions near neutral pH and physiological ionic strength. Here, protein supercharging is used to systematically explore the effect of protein charge on the complex coacervation ...

Potentiometric titration and conformation of synthetic and natural polyelectrolytes.

From the analysis of the potentiometric titration data of polyelectrolytes, we can obtain the electrostatic free energy of the macromolecules. Therefore, potentiometric titration is a useful experimental procedure to study the conformation and conformational change of macromolecules. A few examples of the application of potentiometric titration to the studies on polypeptides, protein and DNA ar...

Monte Carlo study of titration of linear polyelectrolytes

Directed motion of proteins along tethered polyelectrolytes.

We present the first time-resolved investigation of motions of proteins in densely grafted layers of spherical polyelectrolyte brushes. Using small-angle x-ray scattering combined with rapid stopped-flow mixing, we followed the uptake of bovine serum albumin by poly(acrylic acid) layer with high spatial and temporal resolution. We find that the total amount of adsorbed protein scales with time ...

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes.

We have demonstrated a novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid (DNA) sequences, proteins, small molecules, and inorganic ions. This nearly "universal" biosensor approach is based on the observation that, while the conjuga...