Combination of N-acetyl Cysteine and Thymoquinone Alleviates Hepatocellular Toxicities by Radiation and CCl4 Intoxication in SD Rats

Abstract: Radiation and chemicals were the major clinical toxicants known to cause cellular damage during prognostic interventions in vivo. Cellular molecular damages liver causes for hepatocellular injury due various toxicities. Though many antioxidants alleviate types of hepatotoxicities, protection exerted by combination N-acetyl cysteine (NA) Thymoquinone (TQ) combined toxicities radiation carbon tetrachloride (CCLR) Sprague-Dawley (SD) rats unknown. Current research was focused on protective efficacy NA with optimized dose TQ (NATQ) radiation/CCl4 (CCLR)-induced hepatotoxicities. At end experimental period (6 weeks), body weight, serum tissues analyzed marker enzymes (AST, ALT, LDH), oxidative stress level (MDA, GSSG), antioxidant status (GSH, Vitamin E, C), activities enzymatic (SOD, GPx, CAT, GST), histopathology studies hepatic levels NfkB, IL-6, TNF-α, MMP-3, MMP-12, Nrf2 HO-1 done. Significant (p≤0.05) toxicological alterations above parameters recovered normal treatment NATQ combinations SD rats. In conclusion, we provide evidence alleviating hepatotoxicities produced CCLR Keywords: cysteine, Thymoquinone, Carbon tetrachloride, toxicity, MMP.