Colorimetric Determination of Serum Isocitric Dehydrogenase

Colorimetric determination of serum isocitric dehydrogenase. Comparison with a spectrophotometric procedure.

ISOCITRIC DEHYDROGENASE (lCD)5 is one of the newer enzymes that has recently come to the attention of clinical medicine. Sterkel et at. (1) have shown that serum lCD is elevated in hepatitis and to a lesser extent in some malignancies. Bowers and McDuffee (2) observed marked elevations of serum lCD in 2 patients who at autopsy showed extensive central lobular liver cell necrosis. The enzyme act...

Serum transaminases and isocitric dehydrogenase in kwashiorkor.

Serum glutamic oxalacetic transaminase (G-OT), glutamic-pyruvic transaminase (G-PT), and isocitric dehydrogenase (ICD) have been estimated in six groups of subjects, namely, normal British adults, normal Nigerian adults, undernourished Nigerian adults and children, Nigerian children with florid protein malnutrition (kwashiorkor), and cases of protein malnutrition after treatment. The normal ran...

Colorimetric Determination of Serum

In the past few years there has been renewed interest in the study of serum cholinesterase in various disease states, particularly those affecting the liver. Recently, a colorimetric method for the determination of this enzyme in the serum was reported by Ravin, Tsou, and Seligman (1). Their method, simple to perform, is as accurate as the gastrometric technique and more specific than the elect...

Colorimetric vs enzymatic determination of serum phosphorus.

Improved colorimetric determination of serum zinc.

We show how zinc may easily be quantified in serum by first using an optimum concentration of guanidine hydrochloride to cause release of zinc from proteins, followed by complexation of released metals with cyanide. The cyanide complex of zinc is preferentially demasked with chloral hydrate, followed by a colorimetric reaction between zinc and 4-(2-pyridylazo)resorcinol. This is a sensitive wat...