Colored point spread function engineering for parallel confocal microscopy

Point spread function engineering in confocal scanning microscopy

Confocal scanning microscopes are imaging systems that are mainly featured by their unique depth-discrimination capacity when imaging three-dimensional objects. Along the past few years, our research group has done several attempts to improve their axial resolution by means of the so-called point-spread-function (PSF) engineering method. That is, by designing diffractive elements that properly ...

Multicolour localization microscopy by point-spread-function engineering.

Super-resolution microscopy has revolutionized cellular imaging in recent years1-4. Methods relying on sequential localization of single point emitters enable spatial tracking at ~10-40 nm resolution. Moreover, tracking and imaging in three dimensions is made possible by various techniques, including point-spread-function (PSF) engineering5-9 -namely, encoding the axial (z) position of a point ...

Optimized pupil-plane filters for confocal microscope point-spread function engineering.

We present a new method of superresolving pupil-plane filter design in confocal microscopy in which we specify the properties of the desired point-spread function and use an optimization procedure to determine a suitable pupil-plane filter. A new, flexible method of filter implementation using reconfigurable binary optical elements is described, and experimental results are presented.

Multi-kernel deconvolution applied to confocal fluorescence microscopy with engineered point spread function

Programmable vector point-spread function engineering.

We use two nematic liquid crystal spatial light modulators (SLM's) to control the vector point spread function (VPSF) of a 1.3 numerical aperture (NA) microscope objective. This is achieved by controlling the polarization and relative phase of the electric field in the objective's pupil. We measure the resulting VPSF's for several different pupil field polarization states. By using single fluor...