Colony immunoblot assay for the detection of hemolysin BL enterotoxin producingBacillus cereus
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چکیده
منابع مشابه
Detection of Hemolysin BL Gene of Bacillus cereus Isolates
Hemolysin BL (HBL) is the multi-component enterotoxin causing diarrheal type of Bacillus cereus food poisoning. The hbl operon of B. cereus was divided into 4 coding sequences (CDS) and identified as hblC, hblD, hblA and hblB genes consisting of 1,319, 1,221, 1,128 and 1,401 nucleotides encoding proteins of 439, 406, 375 and 466 amino acids with predicted sizes of 49.3, 43.8, 41.7 and 52.3 kDa,...
متن کاملProduction and characterization of monoclonal antibodies against the hemolysin BL enterotoxin complex produced by Bacillus cereus.
A total of five hybridoma cell lines that produced monoclonal antibodies against the components of the hemolysin BL (HBL) enterotoxin complex and sphingomyelinase produced by Bacillus cereus were established and characterized. Monoclonal antibody 2A3 was specific for the B component, antibodies 1A12 and 8B12 were specific for the L(2) component, and antibody 1C2 was specific for the L(1) protei...
متن کاملSwarming behavior of and hemolysin BL secretion by Bacillus cereus.
An association between swarming and hemolysin BL secretion was observed in a collection of 42 Bacillus cereus isolates (P=0.029). The highest levels of toxin were detected in swarmers along with swarm cell differentiation (P=0.021), suggesting that swarming B. cereus strains may have a higher virulence potential than nonswarming strains.
متن کاملEnterotoxic activity of hemolysin BL from Bacillus cereus.
Bacillus cereus causes exotoxin-mediated diarrheal food poisoning. Hemolysin BL (HBL) is a well-characterized B. cereus toxin composed of three components (B, L1, and L2) that together possess hemolytic, cytotoxic, dermonecrotic, and vascular permeability activities. Here, we show that HBL causes fluid accumulation in ligated rabbit ileal loops at a dose of 5 micrograms of each component per lo...
متن کاملIdentification of hemolysin BL-producing Bacillus cereus isolates by a discontinuous hemolytic pattern in blood agar.
Bacillus cereus causes distinct exotoxin-mediated diarrheal and emetic food poisoning syndromes and a variety of nongastrointestinal infections. Evidence is accumulating that hemolysin BL is a major B. cereus virulence factor. We describe two methods for detection of hemolysin BL in crude samples and on primary culture media. In the first method, the highly unusual discontinuous hemolysis patte...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2004
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2004.tb09744.x