Colony Growth of Human Peripheral White Blood Cells In Vitro

Colony growth of human leukemic peripheral blood cells in vitro.

Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation

Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...

Effect of Myeloma-Derived Microparticles on in Vitro Proliferation and Viability of Alloimmune Human Peripheral Blood Mononuclear Cells

Background: Microparticles (MPs) are small vesicles released from the cell membrane. Accordingly, these contain active molecules to mediate biological processes, including cell proliferation and cell cycle progression. The fusion of myeloma cell lines with immunized B lymphocytes is a critical step of hybridoma technology. MPs modulate lymphoproliferation, thereby facilitating B cell expansion ...

Toxic Effect of Verapamil on Human Peripheral Blood Mononuclear Cells and BALB/c Peritoneal Macrophages, in vitro

Background & Aims: Verapamil as a calcium channel blocker has been broadly used in the treatment of many cardiovascular diseases such as hypertension and arrhythmia. Furthermore, the anti-tumor/ antiinflammatory effects of verapamil have been shown. In the present study, the cytotoxic effect of verapamil on human peripheral blood mononuclear cells (PBMCs) and BALB/c peritoneal macrophges has be...

Some factors influencing granulocytic colony formation in vitro by human white blood cells.

The experiments presented in this paper are essential for CSA production but docuwere designed to characterize the colonymont the importance of neutrophilic granstimulating activity (CSA) from white ulocytes to enhance colony formation. blood cell feeder layers on colony formaPossible differences between colony ention by peripheral blood CFCs in soft agar hancement by granulocytes and red cell ...