Collective diffusion coefficient in polyacrylamide gels.

Translational and collective Diffusion in semi-dilute gels

2014 A comparison is made between measurements of the translational and collective diffusion constants in polyacrylamide-water gels. We conclude that : a) the solvent viscosity does not depend on polymer concentration, and b) scaling exponents can be deduced from dynamic light scattering measurements only after correction for solvent displacement and residual hydrodynamic interactions. LE JOURN...

Collective diffusion of gels in a theta solvent

2014 Experimental measurements are reported of the deswelling process in presence of an external applied pressure of polyacrylamide gels at theta conditions. Analysis of the profile of the concentration gradient enables the swelling pressure as a function of concentration to be deduced as well as the collective diffusion coefficient D. To obtain an explicit expression for D, the polymer-solvent...

Demonstration of rhodanese activity in polyacrylamide gels.

Rhodanese activity from crude extracts of Thiobacillus sp. strain IV-85 was demonstrated in polyacrylamide gels after incubation in the reaction mixture by staining with dichloroindophenol in the presence of methylphenazonium methosulfate. The sensitivity of the staining system was found to be 8 x 10 moles of sulfite.

Steps toward peptide polymerization in polyacrylamide gels

Partitioning and diffusion of proteins and linear polymers in polyacrylamide gels.

The equilibrium partition coefficient (K) and diffusion coefficient (Dgel) of two proteins and two linear polymers were measured as a function of polymer content of a 2.7% cross-linked polyacrylamide (PA) gel. The gel concentration, expressed as a volume percentage of PA in the gel (phi), varied between 0 and 14%. The measurements were made by fluorescence spectroscopy; fluorescent dyes were co...