Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris
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چکیده
منابع مشابه
Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris
The mature cDNA of endochitinase from Trichoderma viride sp. was optimised based on the codon bias of Pichia pastoris GS115 and synthesised by successive PCR; the sequence was then transformed into P. pastoris GS115 via electroporation. The transformant with the fastest growth rate on YPD plates containing 4 mg/mL G418 was screened and identified. This transformant produced 23.09 U/mL of the re...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2013
ISSN: 2045-2322
DOI: 10.1038/srep03043