CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design

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CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design

We have developed a new primer design strategy for PCR amplification of distantly related gene sequences based on consensus-degenerate hybrid oligonucleotide primers (CODEHOPs). An interactive program has been written to design CODEHOP PCR primers from conserved blocks of amino acids within multiply-aligned protein sequences. Each CODEHOP consists of a pool of related primers containing all pos...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 2003

ISSN: 1362-4962

DOI: 10.1093/nar/gkg524