Clostridium difficile toxin B is more potent than toxin A in damaging human colonic epithelium in vitro.
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چکیده
منابع مشابه
Toxin A of Clostridium difficile is a potent cytotoxin.
Clostridium difficile is the cause of antibiotic-associated colitis in humans. The organism produces toxin A, which is generally known as the enterotoxin, and toxin B, which is known as the cytotoxin. Toxin A has been reported to have slight cytotoxic activity; in this study we show that cell lines (F9, OTF9-63, and P19) which express a carbohydrate to which toxin A binds are more sensitive to ...
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Clostridium Difficile Toxin A+B Rapid Test provides a procedure for a qualitative detection of Clostridium difficile toxin A (TcdA) and toxin B (TcdB) in two separate bands. A positive band of either toxin is a sign of an underlying Clostridium difficile infection (CDI), which should attract the attention of the physician. Samples used are liquid or semi-liquid human stool samples. Care must be...
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Epidermal growth factor (EGF) exhibits a cytoprotective effect on gastrointestinal epithelia via a receptor-mediated mechanism. We investigated the effect of EGF on Clostridium difficile toxin A (TxA)- and toxin B (TxB)-induced damage of human colon. Ussing-chambered colonic mucosa was exposed serosally to EGF before and during luminal exposure to TxA and TxB. Resistance was calculated from pot...
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Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combination...
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Toxin B (TcdB) is a major pathogenic factor of Clostridum difficile. However, the mechanism by which TcdB exerts its cytotoxic action in host cells is still not completely known. Herein, we report for the first time that TcdB induced autophagic cell death in cultured human colonocytes. The induction of autophagy was demonstrated by the increased levels of LC3-II, formation of LC3+ autophagosome...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1995
ISSN: 0021-9738
DOI: 10.1172/jci117885