Clostridium difficile PCR Cycle Threshold Predicts Free Toxin
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چکیده
منابع مشابه
Clostridium difficile binary toxin CDT
Binary toxin (CDT) is frequently observed in Clostridium difficile strains associated with increased severity of C. difficile infection (CDI). CDT belongs to the family of binary ADP-ribosylating toxins consisting of two separate toxin components: CDTa, the enzymatic ADP-ribosyltransferase which modifies actin, and CDTb which binds to host cells and translocates CDTa into the cytosol. CDTb is a...
متن کاملGenetic characterization of toxin A-negative, toxin B-positive Clostridium difficile isolates by PCR.
Toxin-specific enzyme immunoassays, cytotoxicity assays, and PCR were used to analyze 48 toxin A-negative, toxin B-positive Clostridium difficile isolates from various geographical sites around the world. All the isolates were negative by the TOX-A TEST and positive by the TOX A/B TEST. A deletion of approximately 1.7 kb was found at the 3' end of the toxA gene for all the isolates, similar to ...
متن کاملClostridium Difficile Toxin A+B Rapid Test
Clostridium Difficile Toxin A+B Rapid Test provides a procedure for a qualitative detection of Clostridium difficile toxin A (TcdA) and toxin B (TcdB) in two separate bands. A positive band of either toxin is a sign of an underlying Clostridium difficile infection (CDI), which should attract the attention of the physician. Samples used are liquid or semi-liquid human stool samples. Care must be...
متن کاملFidaxomicin inhibits toxin production in Clostridium difficile.
OBJECTIVES Fidaxomicin, which was recently approved for the treatment of Clostridium difficile-associated diarrhoea, demonstrates narrow-spectrum bactericidal activity via inhibition of RNA polymerase. In this study we evaluated its inhibitory activity versus C. difficile toxin gene expression and toxin production by quantifying toxin mRNA and protein. METHODS The effects of fidaxomicin, its ...
متن کاملClostridium difficile enterotoxin (toxin A): new results.
JOHN STEPHEN,* SUSAN C. REDMOND,* TIMOTHY J. MITCHELL,* JULIAN KETLEY,* DAVID C. A. CANDY,? DOUGLAS W. BURDONt and RICHARD DANIEL§ *Department of Microbiology. University of Birmingham, P.O. Box 363, Birmingham B15 ZTT, U.K., tlnstitute of Child Health, Francis Road, Edgbaston, Birmingham B16 8ET. U.K. , $Department of Microbiology, General Hospital, Steelhouse Lane, Birmingham B4 6NH U.K. , an...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2017
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.00563-17