Cloning, sequencing, and high expression of the proline iminopeptidase gene from Bacillus coagulans
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منابع مشابه
Cloning, sequencing, and high expression of the proline iminopeptidase gene from Bacillus coagulans.
The gene coding for proline iminopeptidase in Bacillus coagulans was cloned and expressed in Escherichia coli. Nucleotide sequencing revealed an 861-bp open reading frame with an unusual TTG initiation codon, encoding a 287-amino-acid protein. The calculated molecular weight of the product was 32,415. The amino acid sequences of the amino-terminal region and those of some peptide fragments obta...
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The gene for proline iminopeptidase from Lactobacillus delbrueckii subsp. lactis DSM 7290 coding for an enzyme that hydrolyses the synthetic substrate L-prolyl-beta-naphthylamide (Pro-beta NA) was cloned in Escherichia coli. An enzymic plate assay was used to screen for positive clones. The gene, designated pepI, was subcloned into vector pUC18 and sequenced. The nucleotide sequence revealed an...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1992
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.174.24.7919-7925.1992