Cloning, purification and characterization of the ribosomal protein L11 from E. coli
نویسندگان
چکیده
منابع مشابه
Cloning, purification and characterization of the ribosomal protein L11 from E. coli
A high-expression system of L11 was constructed and its interaction with other elements of the ribosome were investigated using physicochemical methods. The gene rplK, coding for the protein L11 from the E. coli 50S ribosomal subunit was amplifyied, cloned and over-expressed. The protein L11 was purified under native and denaturing conditions, refolded and the structures of both proteins were c...
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ژورنال
عنوان ژورنال: American Journal of Molecular Biology
سال: 2011
ISSN: 2161-6620,2161-6663
DOI: 10.4236/ajmb.2011.11005