Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus
نویسندگان
چکیده
منابع مشابه
Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus.
Genes encoding the cytotoxin-hemolysin of Vibrio vulnificus were cloned in Escherichia coli by using the lytic cloning vector, lambda 1059. Subcloning in plasmid pBR325 resulted in the isolation of a 3.2-kilobase DNA fragment containing the cytotoxin gene. By using this fragment as a DNA probe, homologous gene sequences were detected in all 54 V. vulnificus strains studied; homologous sequences...
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A gene (vllY) encoding a novel hemolysin of Vibrio vulnificus CKM-1 has been cloned and sequenced. When the vllY gene was expressed in minicells, a unique peptide of approximately 40 kDa was identified. Subcellular fractionation of Escherichia coli cells carrying the vllY gene indicated that the VllY protein was distributed in both the cytoplasmic and the periplasmic fractions, with the notable...
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In a previous study, we showed that Vibrio vulnificus is a ferrophilic bacterium that requires high levels of available iron for growth. In the present study, we show that iron stimulates, in an unusual manner, the production of cytolysin-hemolysin (VvhA), the most potent exotoxin produced by V. vulnificus. The vvhA gene possesses a putative ferric uptake regulator (Fur)-binding box in the regu...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1985
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.50.3.922-924.1985