Cloning and sequencing of the gene encoding Vibrio cholerae O1 fimbrial subunit (fimbrillin)
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چکیده
منابع مشابه
PCR-MEDIATED CLONING A ND EXPRESSION OF THE GENE FOR THE B-SUBUNIT OF VIBRIO CHOLERAE TOXIN ISOLATED RECENTLY IN IRAN
Knowing the nucleotide sequence of the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of V. cholerae. The resulting amplification product was cloned in a common pUC18 vector. Subsequently, a part of this operon encoding the cholera toxin Bsubunit (CTB) was reamplified and cloned between the BamH1 and EcoR1 sites of the same ...
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Until the discovery of the Vibrio cholerae repeat (VCR), the gene capture and expression systems termed integrons had been typically associated with antibiotic-resistance gene cassettes with usually less than five genes in an array. A method is described for the cloning of the ends of large cassette arrays. Conserved restriction sites within VCRs facilitated the mapping by Southern hybridizatio...
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The structural gene for the Vibrio cholerae leucine aminopeptidase (lap) was cloned and sequenced. The cloned DNA fragment contained a 1,503-bp open reading frame potentially encoding a 501-amino-acid polypeptide with a calculated molecular mass of 54,442 Da. The deduced amino acid sequence of the entire protein showed high homology with the sequence of Vibrio proteolyticus leucine aminopeptida...
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To the Editor: The traditional causative agent of cholera, Vibrio chol-erae O1, has 2 biotypes, classical and El Tor. The current seventh pandemic that began in 1961 and has spread to much of the world is caused by the El Tor biotype. This biotype has replaced the classical biotype responsible for the previous pandemics. The classical and El Tor biotypes are differentiated by phenotypic tests (...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1994
ISSN: 0378-1097
DOI: 10.1016/0378-1097(94)90293-3