Cloning and expression of a functional fucose-specific lectin from an orange peel mushroom, Aleuria aurantia
نویسندگان
چکیده
منابع مشابه
Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin.
The carbohydrate-binding specificity of Aleuria aurantia lectin was investigated by analyzing the behavior of a variety of fucose-containing oligosaccharides on an A. aurantia lectin-Sepharose column. Studies with complex-type oligosaccharides obtained from various glycoproteins by hydrazinolysis and their partial degradation fragments indicated that the presence of the alpha-fucosyl residue li...
متن کاملTitle A Novel Core Fucose - specific Lectin from the Mushroom
Core fucose-specific lectin from Pholiota squarrosa 1 A novel core fucose-specific lectin from the mushroom Pholiota squarrosa* Background: Fuc-α1-6 oligosaccharide has a variety of biological functions. Results: Purification of a novel Fucα 1-6-specific lectin from the mushroom Pholiota squarrosa. Conclusion: The lectin binds only to core α1-6 fucosylated N-glycans and not to the other types o...
متن کاملInfluence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL
Protein-carbohydrate interactions are very often mediated by the stacking CH-π interactions involving the side chains of aromatic amino acids such as tryptophan (Trp), tyrosine (Tyr) or phenylalanine (Phe). Especially suitable for stacking is the Trp residue. Analysis of the PDB database shows Trp stacking for 265 carbohydrate or carbohydrate like ligands in 5 208 Trp containing motives. An app...
متن کاملA novel core fucose-specific lectin from the mushroom Pholiota squarrosa.
Fucα1-6 oligosaccharide has a variety of biological functions and serves as a biomarker for hepatocellular carcinoma because of the elevated presence of fucosylated α-fetoprotein (AFP) in this type of cancer. In this study we purified a novel Fucα1-6-specific lectin from the mushroom Pholiota squarrosa by ion-exchange chromatography and affinity chromatography on thyroglobulin-agarose. The puri...
متن کاملDevelopment and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarkers of hepatocellular carcinoma.
The Aleuria aurantia lectin (AAL) derived from orange peel fungus contains five fucose-binding sites that recognizes fucose bound in α-1,2, α-1,3, α-1,4, and α-1,6 linkages to N-acetylglucosamine and galactose. Recently, we have created several recombinant AAL (rAAL) proteins that had altered binding affinity to fucose linkages. In this report, we further characterize the binding specificity of...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1989
ISSN: 0014-5793
DOI: 10.1016/0014-5793(89)80709-4