Cloning and characterization of a β-amyrin synthase gene from the medicinal tree Aralia elata (Araliaceae)
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چکیده
منابع مشابه
Cloning and characterization of a β-amyrin synthase gene from the medicinal tree Aralia elata (Araliaceae).
Aralia elata is an important medicinal plant in China; it produces large amounts of oleanane type triterpene saponins. A full-length cDNA encoding β-amyrin synthase (designated as AeAS) was isolated from young leaves of A. elata by reverse transcription-PCR. The full-length cDNA of AeAS was found to have a 2292-bp open reading frame, encoding a protein with 763 amino acid residues. The ded...
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Panax japonicus C. A. Mey. is one of the rare traditional Chinese herbal medicines; its main active ingredient is ginsenosides. β-amyrin synthase (βAS) is the rate-limiting enzyme in the biosynthesis pathway of oleanane-type ginsenosides. We cloned for the first time the full-length cDNA of βAS from P. japonicus (GenBank: KP658156) using reverse transcription-polymerase chain reaction (RT-PCR) ...
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Conyza blinii H.Lév. is a widely used medicinal herb in southwestern China. The main pharmacological components of C. blinii are a class of oleanane-type pentacyclic triterpene glycosides known as conyzasaponins, which are thought to be synthesized from β-amyrin. However, no genes involved in the conyzasaponin pathway have previously been identified. Here, we identify an oxidosqualene cyclase (...
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The purpose of this study was to investigate the diversity of endophytic fungi of Aralia elata distributed in Northeast China as well as their capacity to produce saponins. Ninety-six strains of endophytic fungi were isolated, and polymerase chain reaction (PCR) and sequencing were employed to identify the isolates. The saponin concentrations of the culture filtrates of representative strains w...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2012
ISSN: 1676-5680
DOI: 10.4238/2012.august.13.4