Clonal micropropagation of rocket (Eruca sativa Mill.)

Purpose. To optimize the method of clonal micropropagation rocket (Eruca sativa Mill.). Methods. Seeds cultivars 'Znahar' and 'Lybid' were used for introduction into in vitro culture. Solutions sodium hypochlorite (35%), ethanol (70%) HgCl2 (0.2%) sterilization. A 5% solution chloramine served as a control. Sterile material was planted on liquid solid agar nutrient media according to Murashige-Skoog (MS) Hamborg-Eveleg (B5). medium MS supplemented with 0.5 mg/l BAP control at all stages reproduction. 6–benzylaminopurine (BAP), benzylaminopurine (BA), kinetin, IAA, NAA IBA added reproduction rooting. The IAA (0.5 mg/l). Results. Sterilization seeds resulted obtaining 93 90% sterile material; however, no viable found. highest indicators sterility studied varieties noted use solution: 93% 89%. In addition, viability this treatment level 90 85%, respectively. Higher both number shoots (8 6) their height 3 cm) obtained (medium B5 – 5 shoots, 4 cm, respectively). most intensive shoot formation occurred kinetin. particular, medium, variety formed 19 17 shoots; 16 13 At concentration 0.8 mg/l, length 10 8 15 cm 18 same tendency observed when increased 1.2 mg/l. lateral roots varied from 7 11 experimental treatments. Most an case adding NAA, 'Lybid'; increasing 9, Conclusions. worst seed after sterilization (93 0% seeds) best 89% 85% seeds). On different prescriptions, smallest obtained. Also, these treatments, vitrification plants, slow growth insignificant observed. longest root system medium. time, under such conditions, regardless concentration, plants too long that can be injured during planting; therefore, it is more appropriate or combination.