Click chemistry enables preclinical evaluation of targeted epigenetic therapies

3D culture adds an extra dimension to targeted epigenetic therapies

Fibroblasts are found in various proportions across the spectrum of carcinomas, constituting, in many cases, the preponderant cell population of the tumor stroma. Data from co-culture and reconstitution experiments indicate that fibroblasts have a prominent role in defining the rate and extent of cancer progression and metastasis. in a recent issue of Cell Cycle, Fiaschi et al. described a new ...

New Insights into the Androgen-Targeted Therapies and Epigenetic Therapies in Prostate Cancer

Prostate cancer is the most common cancer in men in the United States, and it is the second leading cause of cancer-related death in American men. The androgen receptor (AR), a receptor of nuclear family and a transcription factor, is the most important target in this disease. While most efforts in the clinic are currently directed at lowering levels of androgens that activate AR, resistance to...

Click chemistry with DNAw

Click Chemistry background information

Especially biomolecule labeling requires reaction procedures that can be performed under physiological conditions (neutral pH, aqueous solution, ambient temperature) with low reactant concentrations to ensure non-toxic, low background labeling at reasonable time scales while still preserving biological function. Among the plethora of possible reactions only a few generally fit the necessary rea...

Click Chemistry background information

Especially biomolecule labeling requires reaction procedures that can be performed under physiological conditions (neutral pH, aqueous solution, ambient temperature) with low reactant concentrations to ensure non-toxic, low background labeling at reasonable time scales while still preserving biological function. Among the plethora of possible reactions only a few generally fit the necessary rea...