Clamping the TBP stirrup
نویسندگان
چکیده
منابع مشابه
PCR Clamping 27 PCR Clamping
An efficient, PCR based method for the selective amplification of DNA target sequences that differs by a single base pair is described. The method utilises the high affinity and specificity of PNA for their complementary nucleic acids and that PNA cannot function as primers for DNA polymerases.
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TATA-binding protein (TBP) is an essential factor for eukaryotic transcription. In this study, we demonstrated a mouse cDNA encoding a 21 kDa TBP-like protein (TLP). The TLP ORF, carrying 186 amino acids, covered the entire 180 amino acids of the C-terminal conserved domain of mouse TBP with 39% identity and 76% similarity. Northern blot analysis demonstrated that TLP mRNAs were expressed in va...
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ژورنال
عنوان ژورنال: Nature
سال: 1995
ISSN: 0028-0836,1476-4687
DOI: 10.1038/377480a0