CITRATE FORMATION FROM EXOGENOUS SUBSTRATES BY WASHED HUMAN SPERMATOZOA

Micro-cell chamber and washed human spermatozoa.

With the development of the Makler counting chamber, the reproducibility and accuracy of computer-assisted semen analysis and of manual determination of sperm concentration and sperm motility were significantly enhanced beyond what is possible with other methods, such as the hemocytometer, the Horwell chamber, the Petroff-Hausser chamber, and Chartpak slides. The use of Micro-Cell, a disposable...

Techniques for augmentation of exogenous DNA uptake by ovine spermatozoa

Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and...

Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa

BACKGROUND There has been an ongoing debate in the reproductive field about whether mammalian spermatozoa rely on glycolysis, oxidative phosphorylation or both for their energy production. Recent studies have proposed that human spermatozoa depend mainly on glucose for motility and fertilization but the mechanism behind an efficient glycolysis in human spermatozoa is not well understood. Here, ...

The Detection of Spermatozoa on Washed Forensic Exhibits

techniques for augmentation of exogenous dna uptake by ovine spermatozoa

sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous dna. in the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton x100 and dmso, on exogenous dna uptake by sheep spermatozoa and...