cis-Acting elements that regulate the low-pH-inducible urease operon of Streptococcus salivarius
نویسندگان
چکیده
منابع مشابه
Transcriptional regulation of the Streptococcus salivarius 57.I urease operon.
The Streptococcus salivarius 57.I ure cluster was organized as an operon, beginning with ureI, followed by ureABC (structural genes) and ureEFGD (accessory genes). Northern analyses revealed transcripts encompassing structural genes and transcripts containing the entire operon. A sigma70-like promoter could be mapped 5' to ureI (PureI) by primer extension analysis. The intensity of the signal i...
متن کاملThe pH-dependent expression of the urease operon in Streptococcus salivarius is mediated by CodY.
Urease gene expression in Streptococcus salivarius 57.I, a strain of one of the major alkali producers in the mouth, is induced by acidic pH and excess amounts of carbohydrate. Expression is controlled primarily at the transcriptional level from a promoter, pureI. Recent sequencing analysis revealed a CodY box located 2 bases 5' to the -35 element of pureI. Using continuous chemostat culture, t...
متن کاملRole of VicRKX and GlnR in pH-Dependent Regulation of the Streptococcus salivarius 57.I Urease Operon
Ureolysis by Streptococcus salivarius is critical for pH homeostasis of dental plaque and prevention of dental caries. The expression of S. salivarius urease is induced by acidic pH and carbohydrate excess. The differential expression is mainly controlled at the transcriptional level from the promoter 5' to ureI (p ureI ). Our previous study demonstrates that CodY represses p ureI by binding to...
متن کاملDual functions of Streptococcus salivarius urease.
A urease-deficient derivative of Streptococcus salivarius 57.I was constructed by allelic exchange at the ureC locus. The wild-type strain was protected against acid killing through hydrolysis of physiologically relevant concentrations of urea, whereas the mutant was not. Also, S. salivarius could use urea as a source of nitrogen for growth exclusively through a urease-dependent pathway.
متن کاملcadDX operon of Streptococcus salivarius 57.I.
A CadDX system that confers resistance to Cd(2+) and Zn(2+) was identified in Streptococcus salivarius 57.I. Unlike with other CadDX systems, the expression of the cad promoter was negatively regulated by CadX, and the repression was inducible by Cd(2+) and Zn(2+), similar to what was found for CadCA systems. The lower G+C content of the S. salivarius cadDX genes suggests acquisition by horizon...
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ژورنال
عنوان ژورنال: Microbiology
سال: 2002
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-148-11-3599