Circ-VPS13C enhances cisplatin resistance in ovarian cancer via modulating miR-106b-5p/YWHAZ axis

Introduction Ovarian cancer (OC) is the malignant tumor with highest mortality among gynecological cancers. Chemotherapy resistance a major obstacle to OC therapy. Circular RNAs (circRNAs) play crucial roles in development and chemoresistance. However, role potential mechanism of has-circ-001567 (circ-VPS13C) chemoresistance remain unknown. Material methods The levels circ-VPS13C, miR-106b-5p 14-3-3 zeta (YWHAZ) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot assay. Cell Counting Kit-8 (CCK-8) assay was used assess cell viability calculate half inhibition concentration (IC50) cisplatin (DDP). autophagy-related markers measured apoptosis migration evaluated flow cytometry transwell assay, respectively. binding relationship between circ-VPS13C YWHAZ confirmed dual-luciferase reporter Xenograft performed explore vivo. Results Circ-VPS13C up-regulated, while down-regulated DDP-resistant tissues cells. Knockdown enhanced DDP sensitivity repressing autophagy increased via sponging miR-106b-5p. Moreover, directly targeted YWHAZ. Up-regulation alleviated decrease caused depletion. In addition, silencing decreased Conclusions knockdown through modulation miR-106b-5p/YWHAZ axis, providing new biomarker for improving efficacy chemotherapy.