Circ-CCDC66 upregulates REXO1 expression to aggravate cervical cancer progression via restraining miR-452-5p

Abstract Background Cervical cancer is one most common types among females over the world. While its underlying mechanisms remain unclear. Circ-CCDC66 has been revealed to participate in multiple biological functions, and contribute various diseases’ progression. In current study, we aimed demonstrate role of circ-CCDC66 cervical Methods Real-time quantitative PCR (RT-qPCR) was conducted measure expression circ-CCDC66, miR-452-5p, REXO1 mRNA. Cell fractionation assay RNA fluorescence situ hybridization (FISH) were performed locate cells. account kit 8 (CCK-8) used detect cell proliferation ability. Transwell applied evaluate migration or invasion Bioinformatics analysis, biotinylated pull-down, immunoprecipitation, dual-luciferase reporter assays assess association between miR-452 REXO1. Western blot protein The animal tumor model effect vivo. Results upregulated tissues comparison with normal tissues, correlated later stage larger size. Downregulated inhibited proliferation, migration, invasion. an efficient molecular sponge for negatively regulated miR-452-5p expression. MiR-452-5p directly targeted modulate progression via miR-452-5p. Moreover, downregulated found suppress growth vivo . Conclusion Our results demonstrated circ-CCDC66/miR-452-5p/REXO1 axis progression, might provide novel therapeutic targets clinical intervention.