Chromosomes of multipronuclear zygotes resulting from ICSI
نویسندگان
چکیده
منابع مشابه
The chromosomal complements of multipronuclear human zygotes resulting from intracytoplasmic sperm injection.
Implementation of intracytoplasmic sperm injection (ICSI) in human in-vitro fertilization (IVF) has highlighted the need for information about the risk of nuclear spindle damage caused by this procedure. For this purpose we studied the final products of oocyte meiosis at the first cleavage division of multipronuclear zygotes arising after ICSI, and compared the results with abnormally fertilize...
متن کاملA microsurgical technique for enucleation of multipronuclear human zygotes.
Tripronuclear human zygotes were used to determine the feasibility of an enucleation procedure without the use of cytoskeleton inhibitors to obtain intact cytoplasts for nuclear transfer research. Of 61 tripronuclear human zygotes manipulated, 100% of the zygotes survived after removal of one pronucleus and 90.1% after complete enucleation, proving the reliability of the proposed microsurgical ...
متن کاملChromosomal analysis of blastocyst derived from monopronucleated ICSI zygotes: approach by double trophectoderm biopsy
OBJECTIVE This study aims to increase the knowledge about monopronucleated ICSI-derived blastocysts, analyzing trophectoderm biopsies by aCGH and FISH to evaluate their chromosome constitution. METHODS Fifteen monopronucleated ICSI-derived blastocysts were studied. Double trophectoderm biopsy was performed and analyzed by FISH and aCGH. The blastocysts were classified according to chromosome ...
متن کاملThe parental origin correlates with the karyotype of human embryos developing from tripronuclear zygotes
OBJECTIVE It has previously been suggested that embryos developing from intracytoplasmic sperm-injected (ICSI) zygotes with three pronuclei (3PN) are endowed with a mechanism for self-correction of triploidy to diploidy. 3PN are also observed in zygotes after conventional in vitro fertilization (IVF). The parental origin, however, differs between the two fertilization methods. Whereas the vast ...
متن کاملFeedback control of the metaphase-anaphase transition in sea urchin zygotes: role of maloriented chromosomes
To help ensure the fidelity of chromosome transmission during mitosis, sea urchin zygotes have feedback control mechanisms for the metaphase-anaphase transition that monitor the assembly of spindle microtubules and the complete absence of proper chromosome attachment to the spindle. The way in which these feedback controls work has not been known. In this study we directly test the proposal tha...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1997
ISSN: 0268-1161,1460-2350
DOI: 10.1093/oxfordjournals.humrep.a019591