Chromosome characterization and physical mapping of 18S rDNA in Lilium longiflorum originated interspecific hybrids using combined genomic and fluorescent in situ hybridization

This study was aimed to differentiate parental genomes, examining intergenomic composition, and mapping mitotic metaphase chromosomes by localizing 18S rDNA probes in seven interspecific hybrid progenies that originated from Lilium longiflorum. Since single method of situ hybridization has been widely used lily breeding, while this study, flow cytometry conjunction with genomic fluorescent determine the contribution each parent progenies. A significant variation observed DNA content, chromosome length, loci F1 as compared female male parents. L. longiflorum showed nearly two times higher content than parents × Asiatic progenies, but eight hansonii. Genomic results revealed both contributed an equal number their offspring. Fluorescent had 8, 6 7 parents, i.e., White heaven, Bright tower, respectively, whereas cultivar hansonii 8 12 respectively. Interspecific LA, 10–11 LM hybrids. These cytogenetic implied genetic chromosomal Therefore, combined potential be genome specific time-saving approach breeding could status hybrids origin achieving physical detecting genes different genomes.