Chromatography of Microbial Lipids by Centrifugation Through Microparticulate Gel

Separation of lipids by Silica Gel G column chromatography.

A column chromatographic procedure utilizing Silica Gel C is described for separating lipid components of serum and lipoproteins into individual fractions containing hydrocarbons (I), cholesterol esters (II), triglycerides (111), cholesterol (IV) , free fatty acids (V) , and phospholipids (VI). Silica Gel G required no pretreatment except adjustment of moisture content to 10%. The method afford...

Chromatography of Lipids on Commercial Silica Gel Loaded Filter Paper.

Fractionation of human urine by gel chromatography.

High-resolution anion-exchange chromatography of human urine can resolve more than 150 constituents that absorb ultraviolet light. Prefractionation of urine on Sephadex G.10 helped us identify the constituents and simplified the anion-exchange chromatogram. The material associated with the 13 peaks obtained by gel chromatography was pooled and concentrated into six fractions, which were subsequ...

Refolding proteins by gel filtration chromatography.

We have developed a facile means for the refolding of milligram quantities of purified proteins that employs gel filtration chromatography. We demonstrate by electrophoretic mobility shift and NMR spectroscopy that human ETS-1 protein, bovine ribonuclease A and E. coli integration host factor can be refolded into the native conformation using this technique. We have extended this strategy to th...

Application of Gel Chromatography*

INTRODUCTION The term Gel Chromatography refers to the process of separation of solutes caused by the passage of a solution through a column filled with a porous gel. The dissolved particles may enter the pores fully or partially or they may remain completely excluded. This results in a sorting out of the dissolved particles according to their size, so that the largest particles migrate with th...