Chromatography of lipids on commercial silica gel loaded filter paper

Chromatography of Lipids on Commercial Silica Gel Loaded Filter Paper.

Separation of lipids by Silica Gel G column chromatography.

A column chromatographic procedure utilizing Silica Gel C is described for separating lipid components of serum and lipoproteins into individual fractions containing hydrocarbons (I), cholesterol esters (II), triglycerides (111), cholesterol (IV) , free fatty acids (V) , and phospholipids (VI). Silica Gel G required no pretreatment except adjustment of moisture content to 10%. The method afford...

The separation of esters of choline by filter-paper chromatography.

7. It is concluded that tran8-tran8-muconic acid is probably a true metabolite of benzene and is not produced in vivo from the ci8-ci8-acid. It is suggested that the 68-c68-acid need not be an intermediate in the formation of the tran8-tranm-acid in the animal body. The expense of this work was in part defrayed by a grant from the Medical Research Council. We are grateful to Prof. R. Cruickshan...

Study of phenylketonuria by paper chromatography

Gel permeation chromatography of neutral hydroxy lipids on Sephadex LH-20.

Gel-permeation chromatography on Sephadex LH-20, using ethanol as eluent, permits the resolution of neutral hydroxy lipids according to molecular size. The influence of molecular shape, functional groups, chain lengths, and degree of unsaturation, as well as the effect of the eluent on the elution pattern are discussed. The usefulness of the method for the separation of classes of hydroxy lipid...