Chromatographic Separation on Synthetic Adsorbents

Chromatographic Characterization of Adsorption Properties of Carbon and Silica Adsorbents

Adsorption isotherms and specific retention volumes of benzene on MCM41C16 mesoporous silica and F4O porous carbon were determined at different temperatures by employing inverse gas chromatography. The differential isosteric enthalpies, -∆Hads , and entropies, -∆Sads , of adsorption were calculated from the retention time data and compared with the results obtained from the adsorption second vi...

Chromatographic separation of glycated nucleotides.

A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an a...

Chromatographic Separation of the Diastereoisomers

Reduction of folic acid to the tetrahydro level introduces a second asymmetrical center into the molecule at carbon atom 6.1 The resulting tetrahydrofolate, and subsequent derivatives, prepared chemically consist of dl mixtures that are only 50% active in biological reactions. The need for fully reactive isomers of reduced folates led to the enzymatic synthesis of tetrahydrofolate from 7, %dihy...

Dextran and fructose separation on an SMB continuous chromatographic unit

A dextran/fructose mixture was obtained by fermentation of a sucrose rich media using Leuconostoc mesenteroides NRRL B512(f). The dextran and fructose mixture obtained by fermentation as well as a synthetic mixture of pure components were separated using simulated moving bed (SMB) chromatography. In the case of the synthetic feed, the purity in the raffinate (dextran) and extract (fructose) was...

Phospholipids. 3. On the chromatographic separation of glycerophospholipids.