Chromatographic recovery and purification of natural phytochemicals from underappreciated willow bark water extracts

Willow (Salicaceae) bark contains a variety of antioxidant phytochemicals depending mainly on the hybrid. In this study, hot water extraction willow was coupled with sequential preparative scale chromatography to purify crude extracts well-separated and highly concentrated phytochemical fraction. The pre-equilibrated hydrophilic weak cation exchange resins (with acrylic matrix in Na+ ions form) achieved good separation from monosaccharide fractions. Secondly, individual can be purified based their size hydrophobicity differences, using gel permeation adsorption column chromatography. Surprisingly, G-types sephadex demonstrated an efficient not only monosaccharides but complete between (+)-catechin triandrin. Picein salicin further separated according differences. compounds quickly quantified high performance liquid chromatography, gas high-performance anion-exchange picein triandrin were characterized by one- two-dimensional nuclear magnetic resonance spectroscopy techniques. Additionally, authentic applied confirm identification, quantification purity. technique described here might fast, economical, way large-scale isolation purification bark.