Chromatographic Profiles of Deoxyribonucleic Acid Preparations from Rat and Mouse Tissues
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چکیده
منابع مشابه
Chromatographic profiles of deoxyribonucleic acid preparations from rat and mouse tissues.
Experiments by Bendich et al. (1, 2) and by Polli and Shooter (3, 4) have suggested that the deoxyribonucleic acids might be tissue specific. Bendich et al. (1) found that the DNA of rat spleen, kidney, brain, and intestine could be distinguished by their chromatographic profiles on diethylaminoethyl-cellulose. The chromatographic differences between rat kidney and brain DNA were particularly p...
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1. DNA has been isolated from different mammalian tissues. The DNA preparations were free from RNA, protein and polysaccharides and have a similar range of sedimentation coefficients (approx. 24s). 2. Protein was removed by a two-stage extraction with a phenol-cresol mixture by using a detergent with 4-aminosalicylate in the first stage and sodium chloride in the second. 3. Polysaccharides rema...
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An enzyme system capable of incorporating radioactive thymidine into deoxyribonucleic acid has been demonstrated in extracts of Escherichia coli (l-3) and in homogenates from regenerating rat liver (4). The purpose of this report is to present evidence that the enzymes necessary for the incorporation reactions are present in the high speed supernatant fraction of homogenates from regenerating r...
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1. Evidence is presented for the presence of pyridoxamine phosphate oxidase and a pyridoxal phosphate phosphatase in aqueous extracts of E8cherichia coli. 2. Spectrophotometric methods have been developed for the precise measurement of substrate and product concentrations in both enzyme systems. Specific activities of the order of 10-2 tumole/30 min./mg. ofprotein/ml. ofreaction mixture are rep...
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The apparent turnovers of mitochondrial DNA and of the phospholipids, cardiolipln, lecithin, and phosphatidyl ethanolamine, have been determined simultaneously in the mitochondria of rat liver, kidney, brain, and heart. The turnover rate of mitochondrial DNA in liver was similar to previously reported turnover rates of other mitochondrial components. Mitochondrial DNA has a half-life of 6.7 day...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)76876-8