Chromatographic Analysis of Aflatoxigenic Aspergillus flavus Isolated from Malaysian Sweet Corn

High-performance liquid chromatography (HPLC) provides a quick and efficient tool for accurately characterizing aflatoxigenic non-aflatoxigenic isolates of Aspergillus flavus. This method also quantitative analysis AFs in The method’s recovery was assessed by spiking mixture AF at different concentrations to the testing medium. validity confirmed using strains A. HPLC system, coupled with fluorescence detector post-column photochemical reactor, showed high sensitivity detecting spiked or produced flavus isolates. Recovery from medium 10, 20, 60, 80 ppb found be 73–86% this approach. For AFB1 AFB2, limit detection 0.072 0.062 ppb, while quantification 0.220 0.189 respectively. ranged 0.09 50.68 AFB2 between 0.33 9.23 ppb. findings that six more than acceptable 5 incidence sweet corn higher emphasize need field trials explore their real potential production corn.