Chromatofocusing in the purification of staphylococcal enterotoxin D
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چکیده
منابع مشابه
Chromatofocusing in the purification of staphylococcal enterotoxin D.
A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enteroto...
متن کاملPurification of Type B Staphylococcal Enterotoxin.
Frea, James I. (University of Wisconsin, Madison), Elizabeth McCoy, and F. M. Strong. Purification of type B staphylococcal enterotoxin. J. Bacteriol. 86:1308-1313. 1963.-A procedure for the production and isolation of type B staphylococcal enterotoxin in yields up to 4 mg per liter of toxic culture is presented. Staphylococcus aureus S6 was grown in shake flask cultures at 37 C for 24 hr in a ...
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Enterotoxin E produced by Stu~hyZococcus aureus strain FRI (Food Research Institute)-326 was purified by cation exchange chromatography on carboxymethylcellulose, gel tiltration through superfine Sephadex G-75, and gel filtration in 6 M urea with superfine Sephadex G-75. The purified toxin appears to be nearly homogeneous by paper, starch gel, and polyacrylamide gel electrophoresis and double g...
متن کاملIdentification, purification, and some physicochemical properties of staphylococcal enterotoxin C3.
A third staphylococcal enterotoxin C (C3) has been identified, purified, and characterized. Staphylococcal enterotoxin C3 was identified from a Staphylococcus aureus isolated received from England. The purified toxin was determined by gel permeation chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be a simple protein with a molecular weight of 26,900. The isoelect...
متن کاملChromatofocusing: a new method for purification of staphylococcal enterotoxins B and C1.
A new chromatographic procedure was developed which obtained highly purified preparations of staphylococcal enterotoxins B and C1 in yields of 60% from cultures of Staphylococcus aureus and which is faster than any of the separation methods used previously. The procedure involves chromatography on carboxymethylcellulose, removal of alpha-toxin by adsorption to rabbit erythrocyte membranes, and ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1988
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.26.6.1236-1237.1988