Chromatin condensation modulates access and binding of nuclear proteins

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چکیده

منابع مشابه

Chromatin condensation modulates access and binding of nuclear proteins

The condensation level of chromatin is controlled by epigenetic modifications and associated regulatory factors and changes throughout differentiation and cell cycle progression. To test whether changes of chromatin condensation levels per se affect access and binding of proteins, we used a hypertonic cell treatment. This shift to hyperosmolar medium increased nuclear calcium concentrations and...

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EAST Organizes Drosophila Insulator Proteins in the Interchromosomal Nuclear Compartment and Modulates CP190 Binding to Chromatin

Recent data suggest that insulators organize chromatin architecture in the nucleus. The best studied Drosophila insulator proteins, dCTCF (a homolog of the vertebrate insulator protein CTCF) and Su(Hw), are DNA-binding zinc finger proteins. Different isoforms of the BTB-containing protein Mod(mdg4) interact with Su(Hw) and dCTCF. The CP190 protein is a cofactor for the dCTCF and Su(Hw) insulato...

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Transvection, nuclear structure, and chromatin proteins

ESEARCHERS are inventing and honing the technologies to provide increasingly clear pictures of the organization of the nucleus. One driving issue is the role of chromosome arrangement in gene expression. The intractable nature of this question lies in the fact that it is not yet possible to extract a "chromosome arrangement" and assay it for function. Instead, it must be observed in situ and co...

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Nuclear triiodothyronine-binding protein: partial characterization and binding to chromatin.

Nuclei were prepared by sucrose sedimentation of liver homogenates from rats given (125)I-labeled triiodothyronine in vivo. The nuclear extract obtained by treatment of the nuclear pellet with 0.4 M KCl contains the [(125)I]triiodothyronine that had been injected in vivo bound to protein(s). The triiodothyronine bound to nuclear protein(s) in vivo does not readily exchange with triiodothyronine...

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Nuclear constriction segregates mobile nuclear proteins away from chromatin

As a cell squeezes its nucleus through adjacent tissue, penetrates a basement membrane, or enters a small blood capillary, chromatin density and nuclear factors could in principle be physically perturbed. Here, in cancer cell migration through rigid micropores and in passive pulling into micropipettes, local compaction of chromatin is observed coincident with depletion of mobile factors. Hetero...

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ژورنال

عنوان ژورنال: The FASEB Journal

سال: 2009

ISSN: 0892-6638,1530-6860

DOI: 10.1096/fj.08-128959