Cholesterol improves the transfection efficiency of polyallylamine as a non-viral gene delivery vector

Cationic Liposomes Modified with Polyallylamine as a Gene Carrier: Preparation, Characterization and Transfection Efficiency Evaluation.

Purpose: Cationic polymers and cationic liposomes have shown to be effective non-viral gene delivery vectors. In this study, we tried to improve the transfection efficiency by employing the advantages of both. Methods: For this purpose, modified polyallylamines (PAAs) were synthesized. These modifications were done through the reaction of PAA (15 KDa) with acrylate and 6-bromoalkanoic acid deri...

Cellular Uptake Mechanism of Non-Viral Gene Delivery and Means for Improving Transfection Efficiency

Non-viral delivery systems usually include mechanical, electrical, and chemical methods. Cationic liposomes and cationic polymers are two typical classes of non-viral vectors. Com‐ pared with viral vectors, non-viral ones are considered promising vehicles for gene therapy because of their low toxicity, biocompatibility, and controllability [1, 2], although their low efficacy limits their applic...

in vivo transfection study of chitosan-dna-fap-b nanoparticles as a new non viral vector for gene delivery to the lung

Non-linear pharmacodynamics in a non-viral gene delivery system: positive non-linear relationship between dose and transfection efficiency.

A remarkable non-linearity was found between dose and transfection activities of non-viral gene delivery systems, such as a Lipofectamine/DNA complex and an octaarginine-modified multifunctional envelope-type nano device (R8-MEND). We measured the nuclear delivery of pDNA to distinguish the non-linearity in intracellular pharmacokinetics or pharmacodynamics after transfection with R8-MEND at di...

non-viral transfection methods optimized for gene delivery to a lung cancer cell line

background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...